Emergence of the novel pathogenic coronavirus SARS‐CoV‐2 and its rapid pandemic spread presents challenges that demand immediate attention. Here, we describe the development of a semi‐quantitative high‐content microscopy‐based assay for detection of three major classes (IgG, IgA, and IgM) of SARS‐CoV‐2 specific antibodies in human samples. The possibility to detect antibodies against the entire viral proteome together with a robust semi‐automated image analysis workflow resulted in specific, sensitive and unbiased assay that complements the portfolio of SARS‐CoV‐2 serological assays. Sensitive, specific (...) and quantitative serological assays are urgently needed for a better understanding of humoral immune response against the virus as a basis for developing public health strategies to control viral spread. The procedure described here has been used for clinical studies and provides a general framework for the application of quantitative high‐throughput microscopy to rapidly develop serological assays for emerging virus infections. (shrink)

Thinking about Thinking, by Merl R. Wolfard, is a sign of the times, especially American times. Merl is a research engineer of forty years’ standing with a leaning to philosophy. A self-taught “thinker” or philosopher, he has been thinking about thinking since 1950, and the present volume is the result.

THE RANDLORDS by Geoffrey Wheatcroft. New York: Atheneum, 1986. 314 pp., $17.95. CAPITALISM AND APARTHEID: SOUTH AFRICA, 1910?1984 by Merle Lipton. Totowa, N.J.: Rowman & Allanheld, 1985. 400 pp., $19.95. THE ECONOMICS OF THE COLOUR BAR by W. H. Hutt. London: Institute of Economic Affairs, 1964.